In vitro Cytotoxic Activity of a Sulphated Polysaccharide Ulvan against Human Breast and Glioblastoma Cell line

Authors

  • K. Sonia Department of Chemistry, Queen Mary’s College, Chennai – 600 004, Tamil Nadu, India
  • K.S. Meena Department of Chemistry, Queen Mary’s College, Chennai – 600 004, Tamil Nadu, India
  • S. Alamelu Bai Department of Biochemistry, Asan Memorial College of Arts & Science, Chennai 600 100, Tamil Nadu, India

DOI:

https://doi.org/10.35652/IGJPS.2022.12012

Keywords:

Ulvan, Hemolysis, Erythrocyte morphology, Cytotoxicity activity

Abstract

This study was designed to evaluate the hemolysis, anti-inflammatory and cytotoxicity activity of sulphated polysaccharide ulvan extracted from green seaweed Ulva lactuca (UL). Ulvan was extracted and characterized with UV-Visible Spectroscopy, Fourier transform infrared spectroscopy (FTIR) and Scanning electron microscopy (SEM). Total uronic acid and sulphate content were found to be  19.38% w/w and 22.53% w/w. The biocompatibility of the sulphated polysaccharide ulvan was evaluated via hemolysis, its interaction with erythrocyte structure and anti-inflammatory studies. Further, the polysaccharide was treated for cellular toxicity against human breast cancer (MCF-7) and human glioblastoma cancer (U87) cell lines using the 3-(4, 5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay method. This study indicates that the sulphated polysaccharide ulvan was more suitable for biomedical applications with promising chemo preventive and chemotherapeutic activity. © 2022 iGlobal Research and Publishing Foundation. All rights reserved.

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Published

2022-02-04

How to Cite

Sonia, K., Meena, K., & Alamelu Bai, S. (2022). In vitro Cytotoxic Activity of a Sulphated Polysaccharide Ulvan against Human Breast and Glioblastoma Cell line. Indo Global Journal of Pharmaceutical Sciences (IGJPS), 12, 122–127. https://doi.org/10.35652/IGJPS.2022.12012

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