In vitro Cytotoxic Activity of a Sulphated Polysaccharide Ulvan against Human Breast and Glioblastoma Cell line

Abstract

This study was designed to evaluate the hemolysis, anti-inflammatory and cytotoxicity activity of sulphated polysaccharide ulvan extracted from green seaweed Ulva lactuca (UL). Ulvan was extracted and characterized with UV-Visible Spectroscopy, Fourier transform infrared spectroscopy (FTIR) and Scanning electron microscopy (SEM). Total uronic acid and sulphate content were found to be  19.38% w/w and 22.53% w/w. The biocompatibility of the sulphated polysaccharide ulvan was evaluated via hemolysis, its interaction with erythrocyte structure and anti-inflammatory studies. Further, the polysaccharide was treated for cellular toxicity against human breast cancer (MCF-7) and human glioblastoma cancer (U87) cell lines using the 3-(4, 5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay method. This study indicates that the sulphated polysaccharide ulvan was more suitable for biomedical applications with promising chemo preventive and chemotherapeutic activity. © 2022 iGlobal Research and Publishing Foundation. All rights reserved.