In vitro Inhibitory Effect on Alpha Amylase Enzyme by Polyherbal Dip Tea in Diabetes

Mohsina Patwekar; Aamir  Quazi; Faheem  I.P; Mohammad Ajmal Kamal; Mukim M; Gulzar Ahmed Rather; Abhinandan Patil Patil

doi:10.35652/IGJPS.2022.12018

Authors

Mohsina F.P Department of Pharmaceutical science, BAMU, Aurangabad, India ; Luqman College of Pharmacy , Gulbarga, Karnataka, India https://orcid.org/0000-0002-5043-366X
Aamir Quazi KT Patil college of Pharmacy, Osmanabad, Maharashtra, India https://orcid.org/0000-0002-4263-2677
Faheem I.P. Luqman College of Pharmacy, Gulbarga, Karnataka, India https://orcid.org/0000-0003-0453-8126
Mohammad A. Kamal Institutes for Systems Genetics, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, China; King Fahd Medical Research Center, King Abdulaziz University, Saudi Arabia; Department of Pharmacy, Faculty of Allied Health Sciences, Daffodil International University, Bangladesh; Enzymoics, 7 Peterlee place, Hebersham NSW 2770; Novel Global Community Educational Foundation, Australia https://orcid.org/0000-0003-0088-0565
Mukim M Kota College of Pharmacy, Kota, Rajasthan, India https://orcid.org/0000-0001-7260-686X
Gulzar Ahmed Rather Satyabama Institute of Science and Technology, Chennai, India https://orcid.org/0000-0001-7513-8109
Abhinandan Patil Sanjay Ghodawat University, Kolhapur, Maharashtra, India https://orcid.org/0000-0002-1154-0753

DOI:

https://doi.org/10.35652/IGJPS.2022.12018

Keywords:

Diabetes, Dip Tea, Management, Polyherbal, Treatment

Abstract

Objective: The present study aims to prepare novel polyherbal formulations in a unique and accessible tea bag form and also analyses the antidiabetic potentials in vitro. Material and method: The formulation was made from Ichnocarpus frutescens(IC), Ficus dalhousiae (FD), Creteva magna (CM), Alpinia galanga (AG) and Swertia chirata (SC) herbs and used in this study. Indian medicinal plants i.e., polyherbal were subjected to progressive phytochemical analysis and tests were carried out for the determination of α-amylase inhibition activity. The Phytochemical analysis revealed the presence of Alkaloids, Tannins, Anthraquinone, Flavonoids, Saponin and reducing sugar, as the active compounds. The tea infusions of the aforementioned plants were prepared in different formulations like F1, F2, F3, and F4. The formulations were tested for antidiabetic activity in vitro through the inhibition of alpha-glycosidase. Results: The F3 (91.6%) and F4 (92.7%) showed the higher inhibition data against porcine pancreatic α-amylase among the Four formulations. The minimum % alpha-amylase inhibition was recorded in F2 (90.4%) among the medicinal active plants studied. Conclusion: Hyperglycaemia level is reduced via a controllable condition of starch breakdown through pancreatic α-amylase inhibitors. Best results were obtained by the infusion containing The F2 formulation which showed significant antidiabetic activity. © 2022 iGlobal Research and Publishing Foundation. All rights reserved.