An Indigenous Study on Malassezial Susceptibility Testing by Selected Plant Extracts

S.R. Suguna; Tessy Anu Thomas; Sharmila Tirumale

doi:10.35652/IGJPS.2022.12033

Authors

Suguna S.R. Department of Microbiology and Biotechnology, JB Campus, Bangalore University. 560056, India
Tessy Anu Thomas Department of Microbiology and Biotechnology, JB Campus, Bangalore University. 560056, India
Sharmila Tirumale Department of Microbiology and Biotechnology, JB Campus, Bangalore University. 560056, India

DOI:

https://doi.org/10.35652/IGJPS.2022.12033

Keywords:

Dandruff, phytochemicals, ketoconazole, Malassezia furfur, Thin layer chromatography

Abstract

The present study was carried out to evaluate the antifungal activity of ten selected plant extracts of Nelumbo nucifera, Senna auriculata, Salvia officinalis, Tagetes erecta, Psidium guajava, Argemone mexicana, Hibiscus sabdariffa, Hemidesmus indicus, Moringa oleifera, and Glycyrrhiza glabra with ketoconazole as the positive reference standard. Their antimalassezial efficacy was tested using different concentrations of extracts in various solvents. The preliminary screening of plant extracts was determined by Kirby Bauer disc diffusion method and among the ten plant extracts, aqueous extract of S. officinalis and ethanol extract of H. sabdariffa extract exhibited potent antifungal activity against Malassezia furfur. The purification and partial characterization of H. sabdariffa and S. officinalis were carried out by Thin layer chromatography (TLC) technique. The Minimum Inhibitory Concentration (MIC) of H. sabdariffa and S. officinalis against M. furfur by Kirby Bauer method was 0.312 mg/mL and 0.625 mg/mL respectively against 50 µg/mL of Ketoconazole which was used as the standard. The phytochemical analysis of S. officinalis and H. sabdariffa revealed the presence of terpenoids, steroids, tannins, and polyphenols of which the concentration of polyphenols and flavonoids were significantly higher in S. officinalis and H. sabdariffa respectively. Thus, the present study concludes that S. officinalis and H. sabdariffa have a potent activity against M. furfur, thereby reducing the prevalence of dandruff, provided the future perspectives of the study that include characterization and cytotoxicity of the compound. © 2022 Caproslaxy Media. All rights reserved.

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